Fig. 3
From: The present and potential future methods for delivering CRISPR/Cas9 components in plants
Mechanism of Cas9/polycistronic-Csy4-gRNA complexes mediated editing of the genome. A CRISPR/Cas9 construct containing promoter (Pro), Cas9, Csy4, terminator (T), and guide RNA (gRNA1, 2, and 3). Construct delivered into plant cells and transcribed into nucleus. The transcript of Cas9 and Cys4 will translated into proteins (nucleases), whereas multicistronic gRNA are separated into individual sgRNAs (dark blue boxes) due to action of Cys4 nucleases (sky blue circles). Further, gRNA1, 2, and 3 scan their respective target sites where Cas9 (green circles) binds and breaks the genome