A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Journal of Genetic Engineering and Biotechnology

Table 2 Effects of different Agrobacterium culture densities and duration of sonication + vacuum infiltration on the survival rate and gus A gene expression in horse gram

Agrobacterium cell density (OD)	Sonication + vacuum (min)	No. of infected seeds	Mean no. of survived plants (%)	Mean no. of plants expressing gus A gene
Control	0	100	94.00 ± 0.58^a	0 ± 0^h
0.3	2.5 + 2.5	100	87.33 ± 0.56^b	7.8 ± 0.51^g
	5.0 + 5.0	100	82.67 ± 0.76^cd	10.0 ± 0.42^f
	7.5 + 7.5	100	68.33 ± 0.95^ef	8.5 ± 0.32^g
0.6	2.5 + 2.5	100	85.00 ± 0.76^bc	16.7 ± 0.31^c
	5.0 + 5.0	100	80.00 ± 0.52^d	20.8 ± 0.40^a
	7.5 + 7.5	100	72.33 ± 0.70^e	14.7 ± 0.69^d
0.8	2.5 + 2.5	100	70.00 ± 0.82^ef	18.2 ± 0.61^b
	5.0 + 5.0	100	65.67 ± 0.75^f	15.0 ± 0.27^d
	7.5 + 7.5	100	52.00 ± 0.77^g	12.0 ± 0.14^e

Mean values of three separate trials (±) with standard errors. In each column, numbers with different letters indicate they are considerably different from each other according to Duncan’s multiple range test at a probability level of 5%