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Table 2 Effects of different Agrobacterium culture densities and duration of sonication + vacuum infiltration on the survival rate and gus A gene expression in horse gram

From: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Agrobacterium cell density (OD)Sonication + vacuum (min)No. of infected seedsMean no. of survived plants (%)Mean no. of plants expressing gus A gene
Control010094.00 ± 0.58a0 ± 0h
0.32.5 + 2.510087.33 ± 0.56b7.8 ± 0.51g
5.0 + 5.010082.67 ± 0.76cd10.0 ± 0.42f
7.5 + 7.510068.33 ± 0.95ef8.5 ± 0.32g
0.62.5 + 2.510085.00 ± 0.76bc16.7 ± 0.31c
5.0 + 5.010080.00 ± 0.52d20.8 ± 0.40a
7.5 + 7.510072.33 ± 0.70e14.7 ± 0.69d
0.82.5 + 2.510070.00 ± 0.82ef18.2 ± 0.61b
5.0 + 5.010065.67 ± 0.75f15.0 ± 0.27d
7.5 + 7.510052.00 ± 0.77g12.0 ± 0.14e
  1. Mean values of three separate trials (±) with standard errors. In each column, numbers with different letters indicate they are considerably different from each other according to Duncan’s multiple range test at a probability level of 5%