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Journal of Genetic Engineering and Biotechnology
Fig. 5 | Journal of Genetic Engineering and Biotechnology

Fig. 5

From: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Fig. 5

Molecular analysis of in planta T1 transgenic horse gram plants. a, b Genomic DNA was isolated from control (CN) and nine putative T1 transgenic horse gram leaf samples and the PCR analysis performed using NPTII (a) and GUS (b) gene-specific primers, respectively. c, d RT-PCR analysis of transgenic horse gram plants using NPTII c and GUSd gene-specific primers, respectively. Total RNA was isolated from control and putative transgenic lines and then converted to the single-stranded cDNA and performed the RT-PCR to study the expression of NPTII and GUS transcript. Note: 796 bp and 1.0 kb PCR products were observed in all transgenic plants except control (CN) using NPTII and GUS gene-specific primer, respectively. M 1.0 kb DNA ladder (Fermentas, Thermo Scientific), CN control plant, H1–H9 different transgenic lines, BP E. coli binary plasmid pCAMBIA2301

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