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Journal of Genetic Engineering and Biotechnology
Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Fig. 2

In planta Agrobacterium-mediated genetic transformation of horse gram (var. Paiyur 2). a Germinated horse gram seeds used for the infection of Agrobacterium tumefaciens strain. b 7-day-old acclimatized putatively transformed plant. c 14-day-old acclimatized putatively transformed plant. d 50-day-old acclimatized putatively transformed plant by seedlings co-cultivated with Agrobacterium tumefaciens strain EHA105 harboring pCAMBIA2301 binary plasmid. e Putatively transformed plants shows the flowering. f Pod formation at 55 days. g GUS histochemical staining was performed in T1 horse gram seedlings. h Leaves show GUS expression. i Non-transferred (control) plant leaves show no GUS expression at all

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