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Table 2 Isolates of Fusarium oxysporum, soilborne fungi, and oomycetes used in this study and amplifications by different primer pairs

From: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi

Species of isolates

Host plants

Origin

Others

Designed primer pairs

F1/R12)

F1/R2

F2/R1

F2/R2

F3/R1

F3/R2

F. oxysporum f.sp. lilii

Lily

Netherlands

CBS 1303221)

+ 3)

+

+

–

+

+

F. oxysporum f.sp. gladioli

Gladiolus

Japan

MAFF305610

–

–

–

–

–

–

F. oxysporum f.sp. tulipae

Tulip

Japan

MAFF235105

–

–

–

–

–

–

F. oxysporum f.sp. cucumerinum

Cucumber

Aomori, Japan

–

–

–

–

―

–

–

F. oxysporum f.sp. lycopersici r.1

Tomato

Japan

MAFF103037

–

–

–

–

–

–

F. oxysporum f. sp. lycopersici r.2

Tomato

Japan

MAFF238899

–

–

–

–

–

–

F. oxysporum f.sp. melonis

Melon

Japan

MAFF239211

–

–

–

–

–

–

F. oxysporum f.sp. melonis

Melon

Japan

MAFF305544

–

–

–

–

–

–

F. oxysporum f.sp. fragariae

Strawberry

Akita, Japan

No. 1

–

–

–

–

–

–

F. oxysporum f.sp. fragariae

Strawberry

Akita, Japan

No. 2

–

–

–

–

–

–

Fusarium oxysporum f. sp. radicis-lycopersici

Tomato

Japan

MAFF103044

–

–

–

–

–

–

Fusarium oxysporum f. sp. raphani

Radish

Japan

MAFF103057

–

–

–

–

–

–

Fusarium oxysporum f. sp. spinaciae

Spinach

Japan

MAFF103059

–

–

–

–

–

–

Fusarium oxysporum f. sp. dianthi

Carnation

Japan

MAFF103072

–

–

–

–

–

–

Fusarium oxysporum f. sp. rapae

Turnip leaf

Japan

MAFF240321

–

–

–

–

–

–

Botrytis tulipae

Tulip

Japan

MAFF245223

–

–

–

–

–

–

Botrytis sp.

Korean ginseng

Japan

–

–

–

–

–

–

–

Rhizopus stolonifer

Pandanus sp.

Japan

MAFF238790

–

–

–

–

–

–

Cylindrocarpon destructans

Strawberry

Japan

MAFF306591

–

–

–

–

–

–

Penicillium sp.

Ornithogalum sp.

Japan

–

–

–

–

–

–

–

Rhizoctonia solani AG-4

Unknown

Japan

–

–

–

–

–

–

–

Sclerotium rolfsii

Tomato

Japan

–

–

–

–

–

–

–

Calonectoria ilicicola

Soybean

Japan

–

–

–

–

–

–

–

Verticillium dahliae

Egg plant

Japan

–

–

–

–

–

–

–

Phytophthora nicotianae

Chinese chive

Japan

MAFF238148

–

–

–

–

–

–

Phytophthora cactorum

Strawberry

USA

MAFF306274

–

–

–

–

–

–

Phytophthora sojae

Soybean

Japan

MAFF235802

–

–

–

–

–

–

Pythium dissotocum

Rice

Japan

–

–

–

–

–

–

–

Pythium spinosum

Cucumber

Japan

–

–

–

–

–

–

–

  1. 1)CBS: isolate was provided from Westerdijk Fungal Biodiversity Institute (previously Centraalbureau voor Schimmelcultures, the Netherlands). MAFF isolates were provided from the Genebank of the National Agriculture and Food Research Organization (Tsukuba, Japan)
  2. 2)Pairs of Lhs-F1, -F2, -F3, -R1, and -R2 are listed
  3. 3)+ amplified products were observed, ± faint products were observed, − no amplification was observed