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Table 2 Isolates of Fusarium oxysporum, soilborne fungi, and oomycetes used in this study and amplifications by different primer pairs

From: Development of specific primers for Fusarium oxysporum causing damping off of Lilium formolongi

Species of isolatesHost plantsOriginOthersDesigned primer pairs
F1/R12)F1/R2F2/R1F2/R2F3/R1F3/R2
F. oxysporum f.sp. liliiLilyNetherlandsCBS 1303221)+ 3)++++
F. oxysporum f.sp. gladioliGladiolusJapanMAFF305610
F. oxysporum f.sp. tulipaeTulipJapanMAFF235105
F. oxysporum f.sp. cucumerinumCucumberAomori, Japan
F. oxysporum f.sp. lycopersici r.1TomatoJapanMAFF103037
F. oxysporum f. sp. lycopersici r.2TomatoJapanMAFF238899
F. oxysporum f.sp. melonisMelonJapanMAFF239211
F. oxysporum f.sp. melonisMelonJapanMAFF305544
F. oxysporum f.sp. fragariaeStrawberryAkita, JapanNo. 1
F. oxysporum f.sp. fragariaeStrawberryAkita, JapanNo. 2
Fusarium oxysporum f. sp. radicis-lycopersiciTomatoJapanMAFF103044
Fusarium oxysporum f. sp. raphaniRadishJapanMAFF103057
Fusarium oxysporum f. sp. spinaciaeSpinachJapanMAFF103059
Fusarium oxysporum f. sp. dianthiCarnationJapanMAFF103072
Fusarium oxysporum f. sp. rapaeTurnip leafJapanMAFF240321
Botrytis tulipaeTulipJapanMAFF245223
Botrytis sp.Korean ginsengJapan
Rhizopus stoloniferPandanus sp.JapanMAFF238790
Cylindrocarpon destructansStrawberryJapanMAFF306591
Penicillium sp.Ornithogalum sp.Japan
Rhizoctonia solani AG-4UnknownJapan
Sclerotium rolfsiiTomatoJapan
Calonectoria ilicicolaSoybeanJapan
Verticillium dahliaeEgg plantJapan
Phytophthora nicotianaeChinese chiveJapanMAFF238148
Phytophthora cactorumStrawberryUSAMAFF306274
Phytophthora sojaeSoybeanJapanMAFF235802
Pythium dissotocumRiceJapan
Pythium spinosumCucumberJapan
  1. 1)CBS: isolate was provided from Westerdijk Fungal Biodiversity Institute (previously Centraalbureau voor Schimmelcultures, the Netherlands). MAFF isolates were provided from the Genebank of the National Agriculture and Food Research Organization (Tsukuba, Japan)
  2. 2)Pairs of Lhs-F1, -F2, -F3, -R1, and -R2 are listed
  3. 3)+ amplified products were observed, ± faint products were observed, − no amplification was observed