Table 1 A list of AFLP primers and adapters sequence
From: Genetic diversity analysis of some Egyptian Origanum and Thymus species using AFLP markers
Name | Sequence (5′-3′) |
|---|---|
MseI forward adapter | GACGATGAGTCCTGAG |
MseI reverse adapter | TACTCAGGACTCAT |
EcoRI forward adapter | CTCGTAGACTGCGTACC |
EcoRI reverse adapter | AATTGGTACGCAGTCTAC |
MseI primer core region (M) | GATGAGTCCTGAGTAA |
MseI pre-selective primer (M+1) | M+C |
MseI selective primers (M+3) | Â |
M+CAA | GATGAGTCCTGAGTAACAA |
M+CAC | GATGAGTCCTGAGTAACAC |
M+CAG | GATGAGTCCTGAGTAACAG |
M+ CAT | GATGAGTCCTGAGTAACAT |
M+CTA | GATGAGTCCTGAGTAACTA |
M+CTC | GATGAGTCCTGAGTAACTC |
M+CTG | GATGAGTCCTGAGTAACTG |
M+CTT | GATGAGTCCTGAGTAACTT |
EcoRI primer core region (E) | GACTGCGTACCAATTC |
EcoRI pre-selective primer (E+1) | E+A |
EcoRI selective primers (E+3) | Â |
E+AAC | GACTGCGTACCAATTCAAC |
E+ACA | GACTGCGTACCAATTCACA |
E+ACC | GACTGCGTACCAATTCACC |
E+ACG | GACTGCGTACCAATTCACG |
E+ACT | GACTGCGTACCAATTCACT |
E+AGC | GACTGCGTACCAATTCAGC |
E+AGG | GACTGCGTACCAATTCAGG |