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Table 4 Effect of metal ions on serratiopeptidase activity

From: Purification, characterization, and structural elucidation of serralysin-like alkaline metalloprotease from a novel source

Metal ions (5 mM) Relative activity before chelation and incubation with metal ions (%) Regaining enzyme activity after chelation with EDTA and incubation with metal ions (%)
Control 100
Enzyme + EDTA (10 mM) 12 ± 0.8
Ca2+ 138 ± 1.7 68 ± 1.6
Mg2+ 92 ± 0.9 18 ± 1.0
Zn2+ 109 ± 1.5 40 ± 1.4
Cu2+ 98 ± 1.1 19 ± 1.0
Na+ 105 ± 1.4 36 ± 1.3
K+ 102 ± 1.3 27 ± 1.1
  1. Each value represents the mean ± SD for three determinations
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