Fig. 1
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs43141-019-0001-8/MediaObjects/43141_2019_1_Fig1_HTML.png)
a Growth of thermophilic bacterial isolates PW1 and PW2 on nutrient agar at 60 °C. b Detection of thermophilic cellulase activity of PW1 and PW2 bacterial isolates. Purified bacterial isolates of PW1 and PW2 were spotted on NA medium supplemented with 1% CMC and incubated at 60 °C for 24 h and flooded with 1% Gram’s iodine. The appearance of clear halo zones around bacterial growth indicates cellulase production by PW1 and PW2. c Amplification of 16S rDNA of PW1 and PW2 by PCR. Lanes 1 and 2: PCR products showing 16S rDNA amplification of PW1 and PW2, respectively. The size of 16S rDNA amplicon (~ 1500 bp) is indicated. M size marker (in kb). d Phylogenetic analysis of 16S rDNA sequences of Bacillus sp. PW1 and Bacillus sp. PW2. Dendrogram of PW1 and PW2 with related thermophiles (> 99% homology) is shown. The Jaccard’s coefficient scale for genetic relatedness is indicated below phylogenetic tree. e Quantitative cellulase assay of bacterial isolates PW1 and PW2