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Journal of Genetic Engineering and Biotechnology
Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India

Fig. 1

a Growth of thermophilic bacterial isolates PW1 and PW2 on nutrient agar at 60 °C. b Detection of thermophilic cellulase activity of PW1 and PW2 bacterial isolates. Purified bacterial isolates of PW1 and PW2 were spotted on NA medium supplemented with 1% CMC and incubated at 60 °C for 24 h and flooded with 1% Gram’s iodine. The appearance of clear halo zones around bacterial growth indicates cellulase production by PW1 and PW2. c Amplification of 16S rDNA of PW1 and PW2 by PCR. Lanes 1 and 2: PCR products showing 16S rDNA amplification of PW1 and PW2, respectively. The size of 16S rDNA amplicon (~ 1500 bp) is indicated. M size marker (in kb). d Phylogenetic analysis of 16S rDNA sequences of Bacillus sp. PW1 and Bacillus sp. PW2. Dendrogram of PW1 and PW2 with related thermophiles (> 99% homology) is shown. The Jaccard’s coefficient scale for genetic relatedness is indicated below phylogenetic tree. e Quantitative cellulase assay of bacterial isolates PW1 and PW2

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