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Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Purification and characterization of thrombin from camel plasma: interaction with camel tick salivary gland thrombin inhibitor

Fig. 3

a Lineweaver–Burk plot relating the reciprocal of the reaction velocity of the purified camel thrombin to the reciprocal of the substrate concentration in μΜ (1/[S]) by using N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide acetate as substrate. The reaction velocity is the change in absorbance at 405 nm per 30 min. b Effect of pH on the camel thrombin activity using N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide acetate as a substrate in 0.05 M Tris–HCl buffer of various pH values

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