Skip to main content

Table 1 Indel percentage, knockout scores, and mutation type in the transformed oil palm tissues predicted by ICE analysis

From: Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

Target gene

SgRNA

Constructs

Sample

Indel (%)

Model fit (R2)

Knockout score

Mutant allele size (bp)

Mutation type

EgFAD2

EgFAD2-T1

EgFAD2-T2

pYLCRISPR/Cas9P35S-H: EgFAD2

P5

21

0.99

21

-105

Mono-allelic

pYLCRISPR/Cas9P35S-H: EgFAD2

P6

19

0.94

12

-304,

-304

Bi-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC4

20

1

20

-105

Mono-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC7

100

0.84

100

 + 1

Mono-allelic

EgFAD2-T2

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC16

81

0.81

80

-20, -28, -23, -14, -12

Multi-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC17

81

0.81

80

-20, -28, -23, -14, -12

Multi-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC18

2

0.96

1

 + 6, -21

Bi-allelic

EgPAT

EgPAT-T1

pYLCRISPR/Cas9PUbi-H: EgPAT

BC1

3

0.94

3

-5, -10

Bi-allelic

pYLCRISPR/Cas9PUbi-H: EgPAT

BC8

3

0.98

3

 + 4, -11, -10

Multi-allelic

pYLCRISPR/Cas9PUbi-H: EgPAT

BC14

11

0.79

11

-1, + 13, -16

Multi-allelic

EgPAT-T2

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC2

3

0.88

3

 + 2, -2

Bi-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC5

1

0.93

1

-10

Mono-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC6

2

0.91

2

 + 2

Mono-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC7

12

0.75

6

 + 18, + 2, -38, -21, -13

Multi-allelic

pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT

AC18

1

0.93

1

-10

Mono-allelic

  1. The samples were labeled with “P” for protoplasts-, “AC” for Agrobacterium-, and “BC” for biolistic-mediated transformations