Target gene | SgRNA | Constructs | Sample | Indel (%) | Model fit (R2) | Knockout score | Mutant allele size (bp) | Mutation type |
---|---|---|---|---|---|---|---|---|
EgFAD2 | EgFAD2-T1 EgFAD2-T2 | pYLCRISPR/Cas9P35S-H: EgFAD2 | P5 | 21 | 0.99 | 21 | -105 | Mono-allelic |
pYLCRISPR/Cas9P35S-H: EgFAD2 | P6 | 19 | 0.94 | 12 | -304, -304 | Bi-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC4 | 20 | 1 | 20 | -105 | Mono-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC7 | 100 | 0.84 | 100 | + 1 | Mono-allelic | ||
EgFAD2-T2 | pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC16 | 81 | 0.81 | 80 | -20, -28, -23, -14, -12 | Multi-allelic | |
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC17 | 81 | 0.81 | 80 | -20, -28, -23, -14, -12 | Multi-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC18 | 2 | 0.96 | 1 | + 6, -21 | Bi-allelic | ||
EgPAT | EgPAT-T1 | pYLCRISPR/Cas9PUbi-H: EgPAT | BC1 | 3 | 0.94 | 3 | -5, -10 | Bi-allelic |
pYLCRISPR/Cas9PUbi-H: EgPAT | BC8 | 3 | 0.98 | 3 | + 4, -11, -10 | Multi-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgPAT | BC14 | 11 | 0.79 | 11 | -1, + 13, -16 | Multi-allelic | ||
EgPAT-T2 | pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC2 | 3 | 0.88 | 3 | + 2, -2 | Bi-allelic | |
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC5 | 1 | 0.93 | 1 | -10 | Mono-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC6 | 2 | 0.91 | 2 | + 2 | Mono-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC7 | 12 | 0.75 | 6 | + 18, + 2, -38, -21, -13 | Multi-allelic | ||
pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT | AC18 | 1 | 0.93 | 1 | -10 | Mono-allelic |