Fig. 6

Genome editing in oil palm uses the inference of CRISPR Edits (ICE) to estimate the editing efficiency. The ICE indel profiles using dual EgFAD2 sgRNAs are represented graphically for two samples of edited protoplast cells transfected with pYLCRISPR/Cas9PUbi-H: EgFAD2 and two samples regenerated from Agrobacterium-mediated transformation with pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT. (a) The summary results for four samples that were edited by two sgRNAs, including indels and the percent of sequences that are putative knockouts (KO score). (b) The contributions tab shows indels and sequence lists from the dual-sgRNA-edited samples. The black vertical dashed lines represent the cleavage site matched with the site of the most upstream cut for the multiplex sample. The sequence plot represents mutations with fragment deletion and insertion, respectively. The contributions tab shows the inferred sequences and their relative proportions in an edited population, with the “ + ” symbol representing a wild type of sample