Skip to main content
Fig. 5 | Journal of Genetic Engineering and Biotechnology

Fig. 5

From: Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

Fig. 5

Confirmation of CRISPR/Cas9 transformation in oil palm. (a) Hygromycin selection of CRISPR/Cas9 transformed embryogenic calli and (b) plantlet regeneration. Gel electrophoresis of PCR amplicons using specific primers. (c) FAD2-R and FAD2-F primer sets were used to amplify PCR products from transfected protoplasts with pYLCRISPRCas9P35S-H: EgFAD2. Lanes 5 and 6 are positive samples; P5 and P6 are marked with a “ + .” (d) PCR products from embryogenic calli regenerated by Agrobacterium-mediated stable transformation with pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT using FAD2-F and FAD2-R primers. Positive samples of AC4 are found in lanes 4, 7, 16, 17, and 18. Positive samples of AC7, AC16, AC17, and AC18 are marked with a “ + .” (e) PCR products for embryogenic calli derived from Agrobacterium-mediated transformation with the pYLCRISPR/Cas9PUbi-H: EgFAD2EgPAT using EgPAT primer pairs. Lanes 2, 5, 6, 7, and 18 are positive samples of AC2, AC5, AC6, AC7, and AC18, respectively; and (f) PCR products for the regenerated embryogenic calli bombarded with the pYLCRISPRCas9PUbi-H: EgPAT plasmid using the EgPAT-F and EgPAT-R primers; lanes 1, 8, and 14 are positive samples of BC1, BC8, and BC14

Back to article page