Fig. 1

Schematic representation of CRISPR/Cas9-mediated targeted mutagenesis in the oil palm EgFAD2 and EgPAT genes (a). The simplified metabolic pathway for the biosynthesis of fatty acids C16:0 and C18:0 in oil palm PAT and FAD2 genes were knocked out (X) to prevent palmitate from being diverted to TAG and oleate from being desaturated into linoleic acid. The target genes’ sgRNA target sites are depicted in a schematic diagram. Schematic diagram of the targeting sequence (b) Two sgRNAs (T1 and T2) of the EgFAD2 gene correspond to locations in the coding region, and (c) two sgRNAs (T1 and T2) of the EgPAT gene match to first and second exon locations, respectively. Introns are shown as pink boxes, and exons are shown as grey and blue boxes