Fig. 3

Schematic representation of an expression module for a cold-shock vector. Cold-shock proteins (CSPs) are found in all organisms. The promoters of upregulated csp genes, ribosome binding site (RBS), and transcription terminator are the primary genetic elements regulating cold shock-directed expression. The two former elements are sited on the upstream csp 5′-end, whereas the third element is sited downstream on the csp 3′-end. In addition to these genetic elements, the expression module must also contain the translation start codon (ATG), the multiple cloning sites (MCS), and the translation stop codon (Stop). Optionally, it can have some tags, such as the 6xHis tag, to facilitate the purification of recombinant proteins. csp 5′-UTR: 5′-untranslated region of csp, csp 3′-UTR: 3′-untranslated region of csp. Adapted from Bjerga and Williamson 2015