Fig. 4

The schematic view of multiplexed shRNA-miRs (note that the amount and combination of shRNA are flexible) and the mechanism of it in silencing genes. Env-pseudotyped lentiviral vector containing multiplex shRNA-miRs integrates with CD4 T cell, transducing the miRNA-based shRNA into the nucleus. It is then transcribed into primary miRNA that is further cut into precursor miRNA and being transported out to the cytoplasm. The DICER cuts its loop head, and the produced double-stranded miRNA is unwounded by the RISC into a single strand that guides the RISC to cut the target mRNA. Target mRNA will be degraded and results in a gene silencing