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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA

Fig. 3

Expression, purification, and western blotting of chimeric PvpA-pMGA protein. A Electrophoresis of cell lysate of chimeric protein in SDS-PAGE 10% which was induced in different times; Lane 2: cell-lysated from before induction, lanes 3 to 8 are cell-lysated 1 to 16 h after induction. B Electrophoresis of purified recombinant protein. 9: Unstained protein MW marker (Thermo Scientific), lanes 10 to 14 are elutions 1 to 5. C Western blotting of recombinant protein by HRP conjugated anti-His tag antibody in nitrocellulose membrane. Lane 15: prestained protein ladder. Lane 16: purified PvpA-pMGA detected by anti-His-tag antibody

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