Skip to main content

Table 2 Effect of different combinations of γ-radiation (25 and 50 Gy) and Phe (1, 2, 3, and 4 mg/l) on the total phenolic and flavonoid content in the callus cultures of S. marianum L.

From: Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Treatments

Total phenols

(mg/g d.wt.)

Total flavonoids

(mg/g d.wt.)

Control

12.10±0.10d

8.17±0.03bc

Free +1 mg/l Phe

14.21±0.01d

8.61±0.08b

Free +2 mg/l Phe

15.01±0.59cd

8.73±0.15b

Free +3 mg/l Phe

15.33±1.67cd

8.01±0.09c

Free +4 mg/l Phe

19.39±0.10c

7.77±0.14c

25 Gy

18.62±0.07c

8.08±0.10bc

25 Gy + 1 mg/l Phe

23.37±0.12b

9.56±0.12a

25 Gy + 2 mg/l Phe

33.56±0.02ab

8.41±0.09bc

25 Gy + 3 mg/l Phe

34.06±0.03a

8.46±0.04bc

25 Gy + 4 mg/l Phe

34.27±0.02a

7.82±0.08c

50 Gy

18.80±0.07c

7.43±0.06d

50 Gy + 1 mg/l Phe

21.63±0.15bc

8.82±0.07b

50 Gy + 2 mg/l Phe

24.56±0.14b

8.60±0.11b

50 Gy + 3 mg/l Phe

24.81±0.07b

8.16±0.12bc

50 Gy + 4 mg/l Phe

24.94±0.10b

8.01±0.01bc

  1. Different letters indicate a significant difference at P ≤ 0.05