Table 2 Immobilization of partially purified B. subtilis AAK laccase enzyme on different materials
Carrier | Added enzyme (A) | Unbound enzyme (B) | Bound enzyme (I) | Specific activity of the immobilized enzyme (U/mg protein) | Immobilization yield I/(A-B)% |
|---|---|---|---|---|---|
Ionic binding | |||||
 Silica gel | 478.2 | 162.66 | 236.7 | 75.14 | 75.01 |
 Charcoal cubes | 478.2 | 132.06 | 235.98 | 74.91 | 68.17 |
Ionic binding | |||||
 DEAE-cellulose | 478.2 | 69.30 | 268.17 | 85.13 | 65.58 |
Entrapment | |||||
 Alginate 2% | 478.2 | 90.66 | 319.5 | 101.43 | 82.44 |
 Alginate 3% | 478.2 | 97.29 | 356.7 | 113.24 | 93.64 |
 Alginate 5% | 478.2 | 115.95 | 331.05 | 105.10 | 91.39 |