Table 2 List of primers used in SCoT-PCR and ISSR-PCR
SCoT primers | ISSR primers | ||
|---|---|---|---|
Primer name | Sequence (5́′– 3́′) | Primer name | Sequence (5́′›–3́′) |
SCoT-1 | 5′-ACGACATGGCGACCACGC-3′ | ISSR-3 | 5′-ACACACACACACACACYT-3′ |
SCoT-2 | 5′-ACCATGGCTACCACCGGC-3′ | ISSR-4 | 5′-ACACACACACACACACYG-3′ |
SCoT-3 | 5′-ACGACATGGCGACCCACA-3′ | ISSR -5 | 5′-GTGTGTGTGTGTGTGTYG-3′ |
SCoT-4 | 5′-ACCATGGCTACCACCGCA-3′ | ISSR -8 | 5′-AGACAGACAGACAGACGC-3′ |
SCoT-5 | 5′-CAATGGCTACCACTAGCG-3′ | ISSR -9 | 5′-GATAGATAGATAGATAGC-3′ |
SCoT-6 | 5′-CAATGGCTACCACTACAG-3′ | ISSR -13 | 5′-AGAGAGAGAGAGAGAGYT-3′ |
SCoT-7 | 5′-ACAATGGCTACCACTGAC-3′ | ISSR -14 | 5′-CTCCTCCTCCTCCTCTT-3′ |
SCoT-9 | 5′-ACAATGGCTACCACTGCC-3′ | ISSR -19 | 5′-HVHTCCTCCTCCTCCTCC-3′ |
SCoT-10 | 5′-ACAATGGCTACCACCAGC-3′ | ISSR -20 | 5′-HVHTGTGTGTGTGTGTGT-3′ |
SCoT-11 | 5′-ACAATGGCTACCACTACC-3′ | R -9 | 5′- ACACACACACACACACG -3′ |