Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model

Journal of Genetic Engineering and Biotechnology

Table 1 Primer set sequences for the seven virulence and resistance genes

Primer	Sequence	Product size (bp)	Annealing temperature (°C)	References
*ETA*	F: GACAACGCCCTCAGCATCACCAGC	396	60	[40]
*ETA*	R: CGCTGGCCCATTCGCTCCAGCGCT	396	60	[40]
*PhzI*	F: CATCAGCTTAGCAATCCC	392	50	[41]
*PhzI*	R: CGGAGAAACTTTTCCCTC	392	50	[41]
*PhzII*	F: GCCAAGGTTTGTTGTCGG	1,036	51	[41]
*PhzII*	R: CGCATTGACGATATGGAAC	1,036	51	[41]
*bla-TEM*	F: ATGAGTATTCAACATTTCCG	867	50	[42]
*bla-TEM*	R: CTGACAGTTACCAATGCTTA	867	50	[42]
*OprL*	F: CGTGCGATCACCACCTTCTA	171	52.5	Designed for this study
*OprL*	R: CTCGCCCAGAGCCATATTGT	171	52.5	Designed for this study
*ExoS*	F: GCCTTGTCGAGTCCCTTCAA	345	53.5	Designed for this study
*ExoS*	R: GCTTCAGCAGAGTCCGTCTT	345	53.5	Designed for this study
*PlcH*	F: CCTGACTTCGCTGTTCGACT	680	53.5	Designed for this study
*PlcH*	R: CCTGGTTCGGTTCGAGTTCA	680	53.5	Designed for this study