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Table 9 Apigenin and quercitin content as an active compounds identified by HPLC in Dimocarpus longan callus treated by biochemical elicitors and precursor

From: In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Treatments (ppm)

Apigenin content by HPLC mg/g dr.wt

Increase (fold)

Quercitin content by HPLC mg/g dr.wt

Mother plant (derived from tissue culture)

3178.584a

0.00j

N.Dh

Leaf callus (control)

13.204j

0.00j

N.Dh

25 SA

1018.067b

77.09a

N.Dh

50 SA

31.420d

2.379c

18.512a

100 SA

23.227e

1.75d

4.091g

5 MeJA

34.830c

2.637b

N.Dh

10 MeJA

22.357f

1.69e

11.252c

20 MeJA

16.413h

1.24g

12.025b

25 Phe

10.692k

0.80i

7.857e

50 Phe

14.698i

1.11h

5.227f

100 Phe

21.645g

1.63f

9.076d

  1. N.D. not detected
  2. Values followed by the same letter in columns are not different at p < 0.05 by Duncan’s multiple range test, a = Very highly significant (Very high result), b = Highly significant (High result), c = Significant (Intermediate result), than that d-k mean less significant (Less result), and the same letters were not significant