Table 1 Oligonucleotide primers and fluorescent label of loci used in the study
Target | Primer sequence fluorescent label (5′-3′) | Product size bp | Ta°C | Reference |
|---|---|---|---|---|
DXS7424 | F:5′AACACAGGAAGACCCCATC 3′ FAM R:3′GGCTAAGAAGAATCCCGCACA5′ | 76–100 | 58 | [14] |
HPRTB | F:5′TCTCTATTTCCATCTCTGTCTCC3′ FAM R:3′TCACCCCTGTCTATGGTCTCG 5′ | 144–176 | 58 | |
DXS8377 | F: ′5CACTTCATGGCTTACCACAG3′ FAM R:3′GACCTTTGGAAAGCTAGTGT 5′ | 201–261 | 58 | [15] |
GATA31E08 | F:5′CAGAGCTGGTGATGATAGATGA3′ VIC R:3′GCTCACTTTTATGTGTGTATG TATCTCC5′ | 101–129 | 58 | [16] |
DXS7423 | F:5′GTCTTCCTGTCATCTCCCAAC 3′ VIC R:3′TAGCTTAGCGCCTGGCACAT A5′ | 179–191 | 58 | |
DXS8378 | F:5′CACAGGAGGTTTGACCTGTT3′ VIC R:3′AACTGAGATGGTGCCACTGA 5′ | 198–222 | 58 | [14] |
DXS9895 | F: 5′TTGGGTGGGGACACAGAG3′ NED R:3′CCTGGCTCAAGGAATTACAA 5′ | 139–163 | 58 | [16] |
DXS10074 | F 5′ACTTCCTACTGCCCCACCTT3′ NED R:3′GTTTCCCCTCAGAGAGCTGA CACA5′ | 203–227 | 58 | [17] |
DXS6809 | F:5'TGAACCTTCCTAGCTCAGGA3' NED R:3′TCTGGAGAATCCAATTTTGC5′ | 231–278 | 58 | [16] |
DXS7133 | F5′AGCTTCCTTAGATGGCATTCA3′ PET R:3′GTTTTTAACGGTGTTCATGCT T5′ | 80–100 | 58 | |
DXS101 | F:5′ACTCTAAATCAGTCCAAATATCT3′ PET R:3′AAATCACTCCATGGCACATG TAT5' | 197–230 | 58 | |
DXS6807 | F:5′GAGCAATGATCTCATTTGCA3′ PET R:3′AAGTAAACATGTATAGGAAA AAGCT5′ | 250–270 | 58 | [18]. |