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Fig. 9 | Journal of Genetic Engineering and Biotechnology

Fig. 9

From: TRIM21 chimeric protein as a new molecular tool for multispecies IgG detection

Fig. 9

Western blotting detection of CYP1A from protein extracts of mullet fish microsomes. Lane 1, 250 ng of protein extract; lane 2, 125 ng; lane 3, 62.5 ng; lane 4 3.12 ng; lane 5 1.56 ng; lane 6 0.78 ng. Rabbit IgG anti CYP1A was used as the primary antibody at a dilution ratio of 1:7500 in TBS/1% casein. SA-TRIM21 at 0.25 μg/mL was added to the nitrocellulose membrane in TBS/1% casein for rabbit IgG recognition. Biotin HRP (1:2500) was added followed by detection using Luminol based reagent (ECL Scienco Biotech) and chemioluminescent detector

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