Fig. 6
From: TRIM21 chimeric protein as a new molecular tool for multispecies IgG detection
Detection of anti-equine infectious anemia IgG on horse serum. Wells were coated with viral antigens, followed by incubation with horse serum samples. Wells were washed followed by incubation with HRP coupled anti-IgG or SA-TRIM21. After washing, the chromogen TMB was added to the wells followed by incubation during 10 min. Reactions were quenched using Stop solution and absorbance was read at 450 nm