Fig. 2

SA-TRIM21 functional assays. A The performance of streptavidin domain: SA-TRIM21 (0–500 ng) was coated into polystyrene plates followed by detection using Biotin-HRP (1:2500). B Detection of immobilized mouse serum. Mouse serum (50 ng) was coated onto polystyrene plates and detected using HRP-coupled anti-mouse IgG (Sigma, 1:10.000) or 500 ng SA-TRIM21 followed by incubation with biotinylated HRP (dil:1:2500). Control experiments were performed using mouse IgG and biotinylated HRP in the absence of SA-TRIM21. Experiments were performed in triplicates, and error bars represent standard deviation. A, B Wells were washed with PBST, 50 μL chromogenic substrate TMB was added to the wells, incubated for 10 min, followed by the addition of 50 μL H₂SO₄ to quench the reaction followed by OD₄₅₀ nm reading using an ELISA plate reader