Table 2 Oligonucleotid primers designed for real-time PCR
Gene name | Gene symbol | Forward sequence (5′–3′) | Reverse sequence (5′–3′) | Product size (bp) | Connection temperature (°C) |
|---|---|---|---|---|---|
Phosphoenolpyruvate carboxylase | PEPC | GGTGATCCCTACCTGAAGCA | CTCGTGTCCATCACCTCCTT | 152 | 59 |
Isocitrate lyase | TaSAG7 | AAGGCTCCAGTTCGACATCA | TGCTCCAAGGATGAACTGGT | 121 | 59 |
Actin | Â | CCTGTGTTGCTGACTGAGGCCCC | CCAAACGAAGGATAGCATGAGGAAGCG | 232 | 59 |