Table 2 Comparison between next-generation sequencing technologies
From: Bioinformatics approaches and applications in plant biotechnology
Method | Illumina | Pacific Bio | Nanopore | Pyrosequencing (454) | SOLiD |
|---|---|---|---|---|---|
Read length per run | 50–300 base pair | 10–25 kilo base pair | 500–2.3 mega base pair | Approximate 800 base pair | 50 base pair |
Time taken per run | 1 to 10 days | Up to 30 h | 1 min–72 h | 24 h | 1 to 2 weeks |
Cost | $148 per Gb | $2000 Gb | $60–80 per sample | $7000 per sample | $15,000 per 100 Gb |
Accuracy | 98% | 99.9% | 98.9–99.6% | 99.9% | 99.9% |
Advantages | Cost-effective, high-yield sequence reads | Fast, long read lengths | Real-time analysis, long read lengths | Fast, long read lengths | High accuracy |
Disadvantages | Instrument cost, high maintenance of instrument, read length | Low high throughput | Error prone | Homopolymer error | Long run time, low read length |