Table 2 SSR loci primers used on sweet orange genotype studied. The primer sequences are based on Liu et al. [16]
From: A new insight on genetic diversity of sweet oranges: CAPs-SSR and SSR markers
Sequence | Repeat motif | Length | Forward (5′-3′) | Reverse (5′-3′) | GC | TM |
|---|---|---|---|---|---|---|
TAA27 | TAA | 21 | GGATGAAAAATGCTCAAAATG | TAGTACCCACAGGGAAGAGAGC | 57% | 55.69–64.21 °C |
CAC15 | CAC | 22 | TAAATCTCCACTCTGCAAAAGC | GATAGGAAGCGTCGTAGACCC | 40% | 53.11–61.76 °C |
AG14 | AG | 20 | AAAGGGAAAGCCCTAATCTCA | CTTCCTCTTGCGGAGTGTTC | 55% | 54.25–62.72 °C |
CAT01 | CAT | 20 | GCTTTCGATCCCTCCACATA | GATCCCTACAATCCTTGGTCC | 50% | 52.73–61.33 °C |
TC26 | TC | 20 | CTTCCTCTTGCGGAGTGTTC | GAGGGAAAGCCCTAATCTCA | 50% | 51.87–60.61 °C |