Table 1 List of primers used for screening of bcsAB and bcsCD-positive transformants for correct orientation downstream T5 promoter in pQE-Trisystem expression vectors
Clone | Gene ID | Primer | Nucleotide sequence |
|---|---|---|---|
bcsAB | bcsA | pQE-F | 5′- GTTATTGTGCTGTCTCATC-3′ |
bcsA-R | 3′-CGAGGCCGCACGGCTGATCTT-5′ | ||
bcsB | bcsB-F | 5′-AAAATGGTGTCCCTGATCGCGCTG-3′ | |
pQE-R | 5′-ATCGATCTCAGTGGTATTTGTGA-3′ | ||
bcsCD | bcsC | pQE-F | 5′- GTTATTGTGCTGTCTCATC-3′ |
bcsC R | 3′-CTGGTCCATAATAAGATAGTGTGCGGAAATCA-5′ | ||
bcsD | bcsD-F | 5′-ACAACTTTGAACGCAAAACCGGACTTTTCG-3′ | |
pQE-R | 5′-ATCGATCTCAGTGGTATTTGTGA-3′ |