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Table 1 List of primers used for screening of bcsAB and bcsCD-positive transformants for correct orientation downstream T5 promoter in pQE-Trisystem expression vectors

From: Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in Escherichia coli DH5α platform

Clone Gene ID Primer Nucleotide sequence
bcsAB bcsA pQE-F 5′- GTTATTGTGCTGTCTCATC-3′
bcsA-R 3′-CGAGGCCGCACGGCTGATCTT-5′
bcsB bcsB-F 5′-AAAATGGTGTCCCTGATCGCGCTG-3′
pQE-R 5′-ATCGATCTCAGTGGTATTTGTGA-3′
bcsCD bcsC pQE-F 5′- GTTATTGTGCTGTCTCATC-3′
bcsC R 3′-CTGGTCCATAATAAGATAGTGTGCGGAAATCA-5′
bcsD bcsD-F 5′-ACAACTTTGAACGCAAAACCGGACTTTTCG-3′
pQE-R 5′-ATCGATCTCAGTGGTATTTGTGA-3′