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Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in Escherichia coli DH5α platform

Fig. 4

SDS-PAGE (14%) showing the profile of resolved recombinant bcs-proteins in induced cultures of E. coli. The four bcs-recombinant proteins were detected as the expected molecular weights in both B21 (DE3) and DH5α induced cultures (lanes 3 and 4, respectively), but their overexpression was more pronounced in later compared to control uninduced cultures of B21 (DE3) and DH5α (lane 1 and 2, respectively) (A). BC was detected as white gel-like dispersed structures in E. coli DH5α 1 h after IPTG induction (B), and it continued to accumulate in culture media until it was visualized as white filaments 18 h post-induction (C). A less dense BC production was detected in uninduced DH5α cultures suggesting a leaky expression (D)

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