Skip to main content

Table 5 Thin Layer chromatography of methanolic extract of various samples

From: Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

Sample No. of peaks and Rf values
Solvent system 1
Hexane:ethyl acetate (7.2:2.9)
Solvent system 2
Chloroform:acetic acid:methanol:water (6.4:3.2:1.2:0.8)
1 (12) 0.06, 0.10, 0.18, 0.27, 0.41, 0.49, 0.57, 0.60, 0.70, 0.80, 0.89, 1.15 (7) 0.16, 0.23, 0.25, 0.33, 0.44, 0.78, 0.82
2 (14) 0.09, 0.19, 0.24, 0.37, 0.43, 0.51, 0.60, 0.75, 0.79, 0.87, 0.01, 1.12, 1.16, 1.18 (10) 0.9, 0.13, 0.20, 0.24, 0.31, 0.42, 0.52, 0.65, 0.85, 0.91
3 (10) 0.07, 0.17, 0.25, 0.34, 0.42, 0.75, 0.82, 0.87, 1.01, 1.12 (3) 0.43, 0.77, 0.88
4 (13) 0.04, 0.11, 0.18, 0.25, 0.39, 0.43, 0.51, 0.53, 0.75, 0.87, 1.02, 1.08, 1.19 (7) 0.12, 0.16, 0.26, 0.32, 0.43, 0.85, 0.91
5 (10) 0.11, 0.26, 0.35, 0.39, 0.46, 0.58, 0.73, 0.87, 0.98, 1.12 (7) 0.11, 0.17, 0.27, 0.34, 0.43, 0.78, 0.89
6 (11) 0.07, 0.12, 0.18, 0.23, 0.26, 0.33, 0.37, 0.39, 0.76, 0.87, 1.14 (10) 0.60, 0.15, 0.27, 0.34, 0.43, 0.53, 0.69, 0.84, 0.90, 1.05
  1. 1 = tuber (wild), 2 = leaf (wild), 3 = stem (wild), 4 = leaf (in vitro raised), 5 = stem (in vitro raised), 6 = tuber (in vitro raised)