Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

Journal of Genetic Engineering and Biotechnology

Table 2 Effect of selected BAP concentration and different auxin concentrations on shoot multiplication of Dioscorea bulbifera on MS medium

BAP (mg L⁻¹)	IAA (mg L⁻¹)	IBA (mg L⁻¹)	NAA (mg L⁻¹)	Frequency of shoot multiplication (% ± SE)	Shoot number (mean ± SE)	Shoot length (cm) (mean ± SE)
3.5	0.25	⁻	⁻	67 ± 3.33^cd	2.0 ± 0.09^bc	3.3 ± 0.10^cd
3.5	0.50	⁻	⁻	70 ± 5.77^cd	2.5 ± 0.12^acd	3.5 ± 0.05^cd
3.5	0.75	⁻	⁻	87 ± 3.33^abd	3.9 ± 0.21^abd	4.8 ± 0.12^abd
3.5	1.0	⁻	⁻	43 ± 3.33^abc	1.9 ± 0.08^bc	2.4 ± 0.06^abc
3.5	⁻	0.25	⁻	57 ± 3.33	2.0 ± 0.21^b	2.1 ± 0.02^bd
3.5	⁻	0.50	⁻	67 ± 3.33^cd	2.7 ± 0.17^acd	2.9 ± 0.05^acd
3.5	⁻	0.75	⁻	53 ± 6.67^b	2.3 ± 0.03^b	2.1 ± 0.06^bd
3.5	⁻	1.0	⁻	50 ± 5.77^b	2.2 ± 0.10^b	1.5 ± 0.03^abc
3.5	⁻	⁻	0.25	73 ± 3.33^cd	1.3 ± 0.13^bc	3.2 ± 0.02^bcd
3.5	⁻	⁻	0.50	80 ± 5.77^cd	2.0 ± 0.15^a	4.5 ± 0.09^acd
3.5	⁻	⁻	0.75	50 ± 5.77^ab	1.8 ± 0.32^a	2.4 ± 0.11^ab
3.5	⁻	⁻	1.0	43 ± 3.33^ab	1.6 ± 0.19	2.2 ± 0.04^ab

Mean separation was analyzed by ANOVA using SPSS software (16.0) and significance of variations between the concentrations was studied using DMRT at 0.5 % level