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Table 1 Effect of cytokinins on shoot induction from nodal segment explants

From: Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

Concentration of cytokinins (mg L−1)

Frequency of regeneration

(% ± SE)

Shoot number

(mean ± SE)

Shoot length (cm)

(mean ± SE)

BAP

KIN

TDZ

00

0 ± 0.00

0.0 ± 0.00

0.0 ± 0.00

0.5

54 ± 1.85fghi

1.2 ± 0.08efgh

1.2 ± 0.13egh

1.0

54 ± 1.85eghi

1.4 ± 0.12g

1.1 ± 0.22efg

1.5

59 ± 1.85fghi

1.4 ± 0.06g

1.4 ± 0.32egh

2.0

52 ± 1.85cefghij

1.4 ± 0.18g

1.6 ± 0.07egh

2.5

63 ± 1.85abdfghi

1.6 ± 0.18a

2.8 ± 0.20abcdehij

3.0

72 ± 2.99abcdegj

1.7 ± 0.16aij

1.7 ± 0.11be

3.5

87 ± 1.85abcdefhij

2. ± 0.08abcdefhij

3.5 ± 0.04abcdefij

4.0

73 ± 3.33abcdegj

1.7 ± 0.13aij

3.5 ± 0.09abcdefij

4.5

76 ± 1.85abcdeij

1.3 ± 0.06fgh

1.4 ± 0.01egh

5.0

59 ± 1.84dfghi

1.2 ± 0.15fgh

1.5 ± 0.04egh

0.5

41 ± 1.85defgh

1.1 ± 0.08ceg

1.3 ± 0.68bcdeg

1.0

50 ± 3.21deghj

1.3 ± 0.06e

2.8 ± 0.38ahij

1.5

43 ± 1.85degh

1.6 ± 0.06adfhij

2.4 ± 0.17aij

2.0

67 ± 3.21abcefghij

1.2 ± 0.08ce

2.4 ± 0.14aij

2.5

87 ± 1.85abcdfhij

1.7 ± 0.11abdfghij

3.1 ± 0.16afhij

3.0

57 ± 4.90acdeghij

1.2 ± 0.11ce

1.8 ± 0.51e

3.5

85 ± 3.70abcdfhij

1.4 ± 0.05ae

2.6 ± 0.44aij

4.0

76 ± 1.85abcdfgij

1.3 ± 0.06ce

1.7 ± 0.08be

4.5

44 ± 3.21defgh

1.3 ± 0.14ce

1.2 ± 0.07bcdeg

5.0

39 ± 3.21bdefgh

1.2 ± 0.10ce

0.9 ± 0.06bcdeg

0.5

35 ± 1.85c

1.1 ± 0.08bcdehi

0.9 ± 0.06cefghj

1.0

35 ± 1.91c

1.3 ± 0.03a

0.5 ± 0.05dehi

1.5

46 ± 1.93abdgij

1.3 ± 0.05a

0.6 ± 0.05acde

2.0

37 ± 3.70cj

1.5 ± 0.03afg

0.9 ± 0.06bcefghij

2.5

39 ± 3.21j

1.4 ± 0.13afg

1.1 ± 0.05abcdfghij

3.0

41 ± 3.70j

1.1 ± 0.11dehi

0.6 ± 0.09ade

3.5

33 ± 0.00c

1.1 ± 0.11dehi

0.6 ± 0.01ade

4.0

41 ± 3.70j

1.4 ± 0.07afg

0.7 ± 0.03abde

4.5

33 ± 6.41c

1.4 ± 0.07afg

0.7 ± 0.01bdej

5.0

28 ± 3.21cdefh

1.3 ± 0.15

0.5 ± 0.01adei

  1. Mean separation was analyzed by ANOVA using SPSS software (16.0) and significance of variations between the concentrations was studied using DMRT at 0.5 % level