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Table 1 Effect of cytokinins on shoot induction from nodal segment explants

From: Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

Concentration of cytokinins (mg L−1) Frequency of regeneration
(% ± SE)
Shoot number
(mean ± SE)
Shoot length (cm)
(mean ± SE)
BAP KIN TDZ
00 0 ± 0.00 0.0 ± 0.00 0.0 ± 0.00
0.5 54 ± 1.85fghi 1.2 ± 0.08efgh 1.2 ± 0.13egh
1.0 54 ± 1.85eghi 1.4 ± 0.12g 1.1 ± 0.22efg
1.5 59 ± 1.85fghi 1.4 ± 0.06g 1.4 ± 0.32egh
2.0 52 ± 1.85cefghij 1.4 ± 0.18g 1.6 ± 0.07egh
2.5 63 ± 1.85abdfghi 1.6 ± 0.18a 2.8 ± 0.20abcdehij
3.0 72 ± 2.99abcdegj 1.7 ± 0.16aij 1.7 ± 0.11be
3.5 87 ± 1.85abcdefhij 2. ± 0.08abcdefhij 3.5 ± 0.04abcdefij
4.0 73 ± 3.33abcdegj 1.7 ± 0.13aij 3.5 ± 0.09abcdefij
4.5 76 ± 1.85abcdeij 1.3 ± 0.06fgh 1.4 ± 0.01egh
5.0 59 ± 1.84dfghi 1.2 ± 0.15fgh 1.5 ± 0.04egh
0.5 41 ± 1.85defgh 1.1 ± 0.08ceg 1.3 ± 0.68bcdeg
1.0 50 ± 3.21deghj 1.3 ± 0.06e 2.8 ± 0.38ahij
1.5 43 ± 1.85degh 1.6 ± 0.06adfhij 2.4 ± 0.17aij
2.0 67 ± 3.21abcefghij 1.2 ± 0.08ce 2.4 ± 0.14aij
2.5 87 ± 1.85abcdfhij 1.7 ± 0.11abdfghij 3.1 ± 0.16afhij
3.0 57 ± 4.90acdeghij 1.2 ± 0.11ce 1.8 ± 0.51e
3.5 85 ± 3.70abcdfhij 1.4 ± 0.05ae 2.6 ± 0.44aij
4.0 76 ± 1.85abcdfgij 1.3 ± 0.06ce 1.7 ± 0.08be
4.5 44 ± 3.21defgh 1.3 ± 0.14ce 1.2 ± 0.07bcdeg
5.0 39 ± 3.21bdefgh 1.2 ± 0.10ce 0.9 ± 0.06bcdeg
0.5 35 ± 1.85c 1.1 ± 0.08bcdehi 0.9 ± 0.06cefghj
1.0 35 ± 1.91c 1.3 ± 0.03a 0.5 ± 0.05dehi
1.5 46 ± 1.93abdgij 1.3 ± 0.05a 0.6 ± 0.05acde
2.0 37 ± 3.70cj 1.5 ± 0.03afg 0.9 ± 0.06bcefghij
2.5 39 ± 3.21j 1.4 ± 0.13afg 1.1 ± 0.05abcdfghij
3.0 41 ± 3.70j 1.1 ± 0.11dehi 0.6 ± 0.09ade
3.5 33 ± 0.00c 1.1 ± 0.11dehi 0.6 ± 0.01ade
4.0 41 ± 3.70j 1.4 ± 0.07afg 0.7 ± 0.03abde
4.5 33 ± 6.41c 1.4 ± 0.07afg 0.7 ± 0.01bdej
5.0 28 ± 3.21cdefh 1.3 ± 0.15 0.5 ± 0.01adei
  1. Mean separation was analyzed by ANOVA using SPSS software (16.0) and significance of variations between the concentrations was studied using DMRT at 0.5 % level