Table 5 BaGST2 peptides masses measured by HPLC. Peptide masses and their amino acid sequences were obtained by theoretical Lys-C digestion of different GSTs. The amino acid sequences of peptide nos. 3, 9, and 15 were determined using a gas-phase sequencer. The values were rounded to the nearest tenths
Peptide no. | Peptide mass | NM | Position | Peptide sequence | GST source that match to BaGST2 |
|---|---|---|---|---|---|
1 | 890.4 | 1 | 1–8 | MAEAKNVK | Sphingomonas |
2 | 2465.5 | 1 | 9–30 | VLYFDVTGLGEILRLLLKFAGK | B. glabrata |
3 | 1859.9 | 0 | 31–44 | EYEDVRFSFEEWPK | B. glabrata |
4 | 1843.0 | 1 | 47–63 | PTTPFGQMPVLEVDGKK | Copidosoma floridanum |
5 | 1635.9 | 0 | 65–79 | AQSIALAAFLAREFK | B. glabrata |
6 | 2338.2 | 0 | 84–104 | DDLEALQVDATVDTIHDLRAK | Dufourea novaeangliae |
7 | 1065.6 | 0 | 108–115 | SFRESDPVK | B. glabrata |
8 | 1495.5 | 1 | 105–115 | RFKSFRESDPVK | B. glabrata |
9 | 888.6 | 2 | 116–123 | EAIVTEVK | B. glabrata |
10 | 1345.8 | 1 | 130–140 | FMGFFESLLKK | B. glabrata |
11 | 979.5 | 0 | 143–152 | NGSTGLFVGK | Haliotis discus discus |
12 | 1235.7 | 2 | 142–150 | KNGSTGLFVGKK | Haliotis discus |
13 | 1057.5 | 1 | 140–149 | NGGHFVNGKK | Ooceraea biroi |
14 | 1864.0 | 0 | 154–169 | LTWGDFVFAGIYAYLK | Epiphyas postvittana |
15 | 1434.8 | 0 | 170–182 | AAFEAIDNFPLVK | B. glabrata |
16 | 1358.7 | 0 | 183–194 | LVDTVGDNERIK | B. glabrata |
17 | 1234.6 | 1 | 195–206 | WIETRPASKF | Haemonchus contortus |