Table 4 IC50 (μM), Km (μM), Vmax (μmol/min/mg protein), and type of inhibition for BaGST2 and BaGST3 using CDNB as a substrate. The type of inhibition was measured in the presence of less than I20 μM of inhibitor
Inhibitor used | BaGST2 | BaGST3 | ||||||
|---|---|---|---|---|---|---|---|---|
IC50 | Km | Vmax | Type of inhibition | IC50 | Km | Vmax | Type of inhibition | |
Non | - | 0.85 | 75.5 | - | - | 50.0 | 48.8 | - |
BSP | 80.4 | 2.75 | 75.5 | Competitive | 95.0 | 450 | 48.8 | Mixed |
Hematin | 1.35 | 2.54 | 13.7 | Mixed | 0.46 | 50.0 | 15.8 | Noncompetitive |
CB | 2.35 | 3.85 | 75.5 | Competitive | 0.58 | 750 | 48.8 | Competitive |