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Table 3 Km, Vmax (μmol/min/mg protein), pH optima, isoelectric point, IC50, activation energy, and half-life time at 50 °C of BaGST2 and BaGST3

From: Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae)

Enzyme Km (mM) Vmax pH optimum pI IC50 (μM) Activation Energy (kcal/mol) Half-life time at 50 °C
GSH CDNB S-butyl-GSH S–hexyl-GSH S-P-bromobenzyl-GSH No GSH + 5 mM GSH
BaGST2 0.22 3.33 80 > 9.5 4.47 and 4.67 69.1 36.3 9.3 3.75 15 min > 180 min
BaGST3 0.15 and 0.61 0.05 50 6.0 4.17 51.2 19.5 34.9 2.27 25 min > 180 min
  1. Values are means of at least four measurements