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Table 3 Km, Vmax (μmol/min/mg protein), pH optima, isoelectric point, IC50, activation energy, and half-life time at 50 °C of BaGST2 and BaGST3

From: Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae)

Enzyme

Km (mM)

Vmax

pH optimum

pI

IC50 (μM)

Activation Energy (kcal/mol)

Half-life time at 50 °C

GSH

CDNB

S-butyl-GSH

S–hexyl-GSH

S-P-bromobenzyl-GSH

No GSH

+ 5 mM GSH

BaGST2

0.22

3.33

80

> 9.5

4.47 and 4.67

69.1

36.3

9.3

3.75

15 min

> 180 min

BaGST3

0.15 and 0.61

0.05

50

6.0

4.17

51.2

19.5

34.9

2.27

25 min

> 180 min

  1. Values are means of at least four measurements