Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae)

Journal of Genetic Engineering and Biotechnology

Table 1 Purification of GSTs from B. alexandrina snails

Step	Activity (units)	Protein (mg)	Specific activity (units/mg protein)	Purification (fold)	Recovery (%)
Crude extract	54	420	0.13	1.0	100
DEAE cellulose
BaGST₁	4.65	10.6	0.44	3.35	8.6
BaGST2	17.0	32.53	0.52	4.02	31.5
BaGST3	22.4	88.52	0.25	1.95	41.5
GSH-Sepharose
BaGST1	2.93	0.13	22.5	170	5.4
BaGST2	10.09	0.53	19.0	146	20.2
BaGST3	16.73	0.37	45.2	346	31.0