Fig. 4From: Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae)Reverse-phase high-performance liquid chromatography of lysine-C digest of BaGST2. A total of 40 μg of pure sample was reduced with 10 mM DTT at 95 °C, then alkylated in the dark with 20 mM iodoacetamide, and then subjected to SDS-PAGE. After staining and destaining, bands were excised and treated for in situ digestion with lysine-C-specific proteaseBack to article page