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Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: Biochemical characterization and peptide mass fingerprinting of two glutathione transferases from Biomphalaria alexandrina snails (Gastropoda: Planorbidae)

Fig. 4

Reverse-phase high-performance liquid chromatography of lysine-C digest of BaGST2. A total of 40 μg of pure sample was reduced with 10 mM DTT at 95 °C, then alkylated in the dark with 20 mM iodoacetamide, and then subjected to SDS-PAGE. After staining and destaining, bands were excised and treated for in situ digestion with lysine-C-specific protease

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