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Table 1 Recombinant proteins produced at laboratory scale using BEV for diagnosis of COVID-19

From: Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review

Proteins Modifications Host Cells Purification Method Yield Purity Specific Applications References
Moloney MurineLeukemia Virus Reverse Transcriptase (MMLV-RT) TEV-8xHis-Strep tag added Sf9 Ni-NTA affinity chromatography 7.5 mg/l 95% qRT-PCR [15]
Spike Protein, S1 and receptor binding domain. Signal peptides, T4 foldon trimerization domain, cleavage sites and peptide tags added. Mutations introduced to the sequences. Sf9/High Five/Tnao38/Tnms42/Rachiplusia nu larvae Ni-NTA affinity chromatography 0.5 – 1.5 mg/l or 15 ug/g larvae Up to 99% ELISA [27,28,29,30,31]
Spike and nucleocapsid protein Removal of transmembrane and endodomains. Kozak motif, IZN4 fold on trimerization, thrombin cleavage site and hexahistidine tag added. Sf9/High Five Ni-NTA affinity chromatography Not measured Not measured mPLEX-CoV assay 32]