Table 1 Recombinant proteins produced at laboratory scale using BEV for diagnosis of COVID-19
Proteins | Modifications | Host Cells | Purification Method | Yield | Purity | Specific Applications | References |
|---|---|---|---|---|---|---|---|
Moloney MurineLeukemia Virus Reverse Transcriptase (MMLV-RT) | TEV-8xHis-Strep tag added | Sf9 | Ni-NTA affinity chromatography | 7.5 mg/l | 95% | qRT-PCR | [15] |
Spike Protein, S1 and receptor binding domain. | Signal peptides, T4 foldon trimerization domain, cleavage sites and peptide tags added. Mutations introduced to the sequences. | Sf9/High Five/Tnao38/Tnms42/Rachiplusia nu larvae | Ni-NTA affinity chromatography | 0.5 – 1.5 mg/l or 15 ug/g larvae | Up to 99% | ELISA | |
Spike and nucleocapsid protein | Removal of transmembrane and endodomains. Kozak motif, IZN4 fold on trimerization, thrombin cleavage site and hexahistidine tag added. | Sf9/High Five | Ni-NTA affinity chromatography | Not measured | Not measured | mPLEX-CoV assay | 32] |