Molecular characterization of endophytic and ectophytic plant growth promoting bacteria isolated from tomato plants (Solanum lycopersicum L.) grown in different soil types

Journal of Genetic Engineering and Biotechnology

Table 1 Primers used in this study

Target gene	Primers used	sequence	size	Annealing temperature	Reference bacteria	Reference
BOX A1	BOXA1 R	CTACGGCAAGGCGACGCTGACG	Multiple sizes	53 °C	P. fluorescens	[29]
16S rRNA	F-27 R1494-1514	AGA GTT TGA TC (AC) TGG CTC AG CTA CGG (T/C) TAC CTT GTT ACG AC	1400	56 °C	P. fluorescens	[30, 31]
ItuD	ItuD1f ItuD1r	GATGCGATCTCCTTGGATGT ATCGTCATGTGCTGCTTGAG	647	55 °C	B. amyloliquefaciens	[32]
srfC	Sur3f Sur3r	ACAGTATGGAGGCATGGTC TTCCGCCACTTTTTCAGTTT	441	55 °C	B. amyloliquefaciens	[32]