Rational engineering of industrial S. cerevisiae: towards xylitol production from sugarcane straw

Journal of Genetic Engineering and Biotechnology

Table 2 Xylitol productivity of strains FMYX and CENPKX in YPDX under varying cultivation conditions

Experiment	Strain	Varying condition	Xylitol
Experiment	Strain	Varying condition	Titre (g/L)	Yield (g/g)	Productivity (g/L.h)
Co-substrate ratio Fixed 20 g/L xylose; initial OD 0.5 and full aeration	FMYX	10 g/L glucose	13.06 ± 1.03a	0.78 ± 0.06a	0.13 ± 0.01a
		20 g/L glucose	15.60 ± 3.71a	0.80 ± 0.20a	0.15 ± 0.04a
		30 g/L glucose	15.74 ± 0.36a*	0.91 ± 0.02a*	0.15 ± 0.00a*
	CENPKX	10 g/L glucose	14.29 ± 0.89ab	0.83 ± 0.06a	0.14 ± 0.01ab
		20 g/L glucose	14.54 ± 0.09a	0.74 ± 0.00ab	0.14 ± 0.00a
		30 g/L glucose	12.84 ± 0.27b	0.69 ± 0.02b	0.12 ± 0.00b
Aeration Fixed 20 g/L xylose; 30 g/L glucose and initial OD 0.5	FMYX	Aerobic	15.74 ± 0.36a*	0.91 ± 0.02a*	0.15 ± 0.00a*
	FMYX	Semi-anaerobic	10.75 ± 1.51b	0.71 ± 0.04b	0.10 ± 0.01b
	CENPKX	Aerobic	12.84 ± 0.27a	0.69 ± 0.02a	0.12 ± 0.00a
	CENPKX	Semi-anaerobic	9.92 ± 0.39b	0.78 ± 0.05a	0.10 ± 0.00b
Inoculum concentration Fixed 20 g/L xylose; 30 g/L glucose and full aeration	FMYX	OD 0.5	15.74 ± 0.36b*	0.91 ± 0.02a*	0.15 ± 0.00b*
	FMYX	OD 1.0	16.97 ± 0.10a*	0.93 ± 0.02a*	0.16 ± 0.00a*
	CENPKX	OD 0.5	12.84 ± 0.27b	0.69 ± 0.02b	0.12 ± 0.00b
	CENPKX	OD 1.0	15.38 ± 0.24a	0.83 ± 0.01a	0.15 ± 0.00a

Letters (a, b, c) represent statistical differences (Tukey test) observed in different cultivation conditions within the same experiment and strain. Asterisk (*) represents statistical difference (Tukey test) observed between strains within the same experiment condition