Table 1 Main S. cerevisiae strains and plasmids used in this work
From: Rational engineering of industrial S. cerevisiae: towards xylitol production from sugarcane straw
S. cerevisiaestrains | Relevant genotype | Source |
JAY270 | MATa/MATα (PE-2 derived) | (Argueso et al., 2009) [24] |
JAY270ΔURA3 | JAY270 ura3Δ | This work |
JAY270ΔHO | JAY270 hoΔ | This work |
JAYX | JAY270 hoΔ::xyl1 | This work |
FMY001 | MATa/MATα (SA-1 derived) | (de Mello et al. 2019) [22] |
FMY001ΔHO | FMY001 hoΔ | This work |
FMYX | FMY001 hoΔ::xyl1 | This work |
CEN.PK-122 | MATa/MATα MAL2-8c SUC2 | (van Dijken et al. 2000) [25] |
CEN.PK-122ΔHO | CEN.PK-122 hoΔ | This work |
CENPKX | CEN.PK-122 hoΔ::xyl1 | This work |
Plasmids | Relevant feature | Source |
p425_GPD | LEU2 GAPp_CYC1t | (Mumberg et al. 1995) [26] |
p425_XYL1 | LEU2 GAPp_PsXYL1_CYC1t | This work |
pGS | pTEF1-Cas9-tCYC1; pSNR52-sgRNA-tSUP4 | This work |
pGS.29 | gRNA sequence targeting HO in Industrial strains | This work |
pGS.30 | gRNA sequence targeting HO in CEN.PK-122 | This work |