Fig. 2
From: Rational engineering of industrial S. cerevisiae: towards xylitol production from sugarcane straw
Standardization of a CRISPR-Cas9 system for the genome editing of diploid industrial strains with plasmid pGS (Cas9 + sgRNA scaffold), using JAY270 (PE-2 derived) as a proof of concept. A Editing efficiency of the URA3 locus knockout using different concentrations of plasmid pGS and a 90-bp-long donor DNA. B Editing efficiency of the URA3 locus knock-in using different donor overhang homologies to the editing site