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Table 4 The effect of nitrogen source on protease production by Rhizomucor miehei

From: Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation

Type and concentration of nitrogen source

Protein content (mg/mL)

Milk clotting activity (SU/mL)

Specific activity (SU/mg)

Proteolytic activity (PU/mL)

MCA/PA

Blank

2.11 ± 0.01ab

444 ± 19.50a

210.43 ± 7.96a

0.92 ± 0.06a

481.7 ± 11.27a

Yeast extract

 1%

2.11 ± 0.02a

555.42 ± 23.43b

263.23 ± 8.65b

0.94 ± 0.06a

591.45 ± 14.46b

 2%

2.19 ± 0.04bcd

424.14 ± 15.61a

193.67 ± 4.03ac

0.94 ± 0.06a

450.42 ± 19.29ac

 3%

2.13 ± 0.04ab

370.28 ± 9.39c

173.84 ± 1.88c

1.33 ± 0.07b

279.48 ± 8.03d

 4%

2.13 ± 0.05ab

324.32 ± 22.60d

152.26 ± 7.82d

1.38 ± 0.08b

243.92 ± 5.81e

 5%

2.10 ± 0.04a

231.75 ± 20.41e

110.36 ± 7.83e

1.41 ± 0.10b

160.89 ± 7.40f

Peptone

 1%

2.11 ± 0.03ab

571.42 ± 19.51b

270.82 ± 7.09bf

1.46 ± 0.10b

392.04 ± 15.20g

 2%

2.13 ± 0.03ab

648.64 ± 27.44fg

304.53 ± 8.61gh

2.34 ± 0.10dc

278.75 ± 14.31d

 3%

2.14 ± 0.04ab

666.66 ± 24.57f

311.52 ± 6.72g

2.46 ± 0.20d

272.23 ± 24.91d

 4%

2.12 ± 0.04ab

613.89 ± 20.42g

289.57 ± 4.87fhkm

1.92 ± 0.30e

320.51 ± 15.35h

 5%

2.08±0.04a

571.42 ± 18.35b

274.72 ± 3.90bk

1.30 ± 0.16b

438.79 ± 9.88c

Casein

 1%

2.23±0.04c

827.58 ± 20.25h

371.11 ± 3.59n

1.40 ± 0.07b

591.46 ± 23.51b

 2%

2.27±0.06c

857.14 ± 20.81h

377.59 ± 1.91n

1.62 ± 0.09be

527.76 ± 20.62i

 3%

2.38±0.05e

666.66 ± 23.61f

280.11 ± 4.63g

2.02 ± 0.10ce

328.87 ± 11.84h

 4%

2.24±0.03cd

666.66 ± 19.11f

297.62 ± 4.25g

5.26 ± 0.27f

127.27 ± 3.96j

 5%

2.15±0.03ad

648.64 ± 19.42fg

301.69 ± 4.95gm

5.98 ± 0.32g

108.62 ± 2.49j

  1. The results are expressed as mean ± SD. Within columns, values followed by the same letter(s) are not significantly different at the 0.01 level