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Table 3 The effect of medium formulation on protease production by Rhizomucor miehei

From: Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation

Medium formulation

Protein content (mg/mL)

Milk clotting activity (SU/mL)

Specific activity (SU/mg)

Proteolytic activity (PU/mL)

MCA/PA

Wheat bran

1.44 ± 0.04a

364.6 ± 30.94a

253.19 ± 14.33a

1.2 ± 0.06a

301.81 ± 9.30a

Wheat bran replaced with corn powder

 10%

1.36 ± 0.04b

314.66 ± 9.24b

231.36 ± 0.05b

1.44 ± 0.13ab

220.03 ± 12.63b

 20%

1.32 ± 0.01b

296.44 ± 11.4bc

213.15 ± 6.77b

1.38 ± 0.10ac

216 ± 7.41b

 30%

1.16 ± 0.01c

275.8 ± 5.72cde

237.75 ± 3.14ab

1.61 ± 0.17bcd

172.19 ± 13.37c

 40%

1.14 ± 0.01cd

265.09 ± 6.03dh

232.27 ± 3.86b

1.21 ± 0.10a

219.99 ± 14.02b

Wheat bran replaced with wheat flour

 10%

1.44 ± 0.03a

295.33 ± 11.48be

205.09 ± 3.63c

2.08 ± 0.25be

142.16 ± 10.72d

 20%

1.18 ± 0.01c

221.23 ± 12.71fg

187.09 ± 8.87e

1.28 ± 0.05ad

172.92 ± 6.45c

 30%

1.1 ± 0.01de

206.89 ± 3.57gi

188.12 ± 2.44e

2.64 ± 0.20f

78.64 ± 5.01ef

 40%

1.08 ± 0.01e

205.13 ± 14.55gi

189.52 ± 11.88ce

1.5 ± 0.17ad

136.84 ± 7.52d

Wheat bran replaced with soybean powder

 10%

1.52 ± 0.02f

237.95 ± 9.9hf

156.54 ± 4.93f

2.47 ± 0.22f

96.24 ± 4.83f

 20%

1.58 ± 0.01g

198.2 ± 7.31gi

125.44 ± 4g

2.72 ± 0.24f

72.99 ± 3.82e

 30%

1.78 ± 0.004h

192 ± 6.78ij

108.18 ± 3.57h

2.71 ± 0.10f

71.01 ± 0.19e

 40%

1.52 ± 0.003f

169.04 ± 7.37j

111.21 ± 4.65gh

1.9 ± 0.08de

88.94 ± 0.94ef

  1. The results are expressed as mean ± SD. Within columns, values followed by the same letter(s) are not significantly different at the 0.01 level