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Fig. 7 | Journal of Genetic Engineering and Biotechnology

Fig. 7

From: Expression and characterization of hemagglutinin–neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800

Fig. 7

The result of western blotting to increase the sensitivity in the detection of membrane proteins. Lane 1: marker weight (#SL7001-Sinaclon, Iran). Lane 2: pre-induced protein without expression. Lane 3: a positive control sample, a positive western bloat response to a His-Tag protein sample weighing 63 kD. Lane 4: confirmation of recombinant HN protein (63KD) expression, 8 h after induction. Lane 5: lack of recombinant protein secretion in bacterial cells containing plasmid without HN genes

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