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Journal of Genetic Engineering and Biotechnology
Fig. 6 | Journal of Genetic Engineering and Biotechnology

Fig. 6

From: Expression and characterization of hemagglutinin–neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800

Fig. 6

Evaluation of recombinant protein expression in B. subtilis. A Summary of the normalized quantities of expression proteins in three bacterial groups. One-way analysis of variance (ANOVA) by Tukey’s method showed that in level P ≤ 0.05, the observed difference in pHT43_NH expression IPTG-induced pHT43 and the B. subtilis WB600 groups is statistically significant, whereas there was no statistically significant relationship between pHT43 and B.subtilis WB600. B Investigation of expression and secretion of recombinant HN protein (63KDa) in culture medium with acrylamide gel 12%. Column 1: expression before induction. Columns 2 to 5: gradual increase in the expression from 1 to 8 h after induction. Column 6: molecular weight (#SL7001-Sinaclon, Iran). Column 7: lack of protein secretion in the culture medium before induction. Column 8: lack of recombinant protein secretion in bacterial cells containing plasmid without HN genes

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