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Journal of Genetic Engineering and Biotechnology
Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Expression and characterization of hemagglutinin–neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800

Fig. 2

Enzymatic digestion map of the new pGet-HN recombinant T/A cloning vector. A Lane 1: BamHI/Xb double digestion on the HN-containing recombinant T/A cloning vector (clone) revealed expected 3954-bp and 1734-bp fragments. Lanes 2–5: single digested, revealed expected fragment (5688 bp). Lane 6: 1-kb DNA size marker (Fermentas #SM0311). Lane 7: undigested plasmid of the new HN-containing recombinant T/A cloning vector. B HN, gene PCR with specific primer, six white colonies were selected randomly. Lane 1: negative control (blank). Lane 2: 1-kb DNA size marker (Fermentas #SM0311). Lanes 3–8: HN-gene fragment (1734 bp)

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