Table 2 Characteristics of the selected coconut SSRs with name, primer sequence, annealing temperature, repeat motif, contig number, and expected allele

1

CNMK7

F: GAGGGAGAGAGGCTGTGATG

R: GCATGTTCAAGGCTTTGGTT

55.6

56

299

AG

0

242–260; 404–480

P

2

2

CNMK172

F: TTTCCCATCTTGATCCACCT

R: TTTGATGGCTGGTGTAATGC

53.55

57

280

TG

1

280–320

P

3

3

CNMK329

F: AGTGGCTTCAAGTGGGTCAG

R: CACCATCCTTGGCTTTCAAT

55.3

55

342

CT

1

320–360

P

2

4

CNMK653

F: AGCCCAAATTCGATCAACTG

R: ATACATGGCAATGGCACCTT

54.15

57

240

GA

4

130–170; 170–230

P

3

5

CNMK765

F: AATGTTATGCGAACCCTTGC

R: TGACAAGGTGGACACTTGGA

55

53

211

GA

5

210–265

P

4

6

CNMK995

F: AAGACACGACACCCGATACC

R: AAGTGCAACAGCACAACTGG

56.55

54

196

AG

6

190–242

P

2

7

CNMK1095

F: CCTCATCGGCTAACCTCAAG

R: AGTCCTGAACCGAGGTACGA

56.25

54

229

CT

7

230–250

P

4

8

CNMK3414

F: CCAGCTCACTGCCATACAGA

R: AGCACCAGGCTCTCATCTTC

56.85

50

289

AC

35

290–380

P

2

9

CNMK3683

F: ACCTCCCAGTGAAGACATGC

R: CCGTTACGTTATCCCTTCCA

55.65

54

360

TG

40

320–370

P

2

10

CNMK4036

F: TGAGTTTCCTGGACCAAAGC

R: ATACGCTGCTAGGGTGGTGT

56.8

55

254

GA

45

290–320

P

3

11

CNMK4153

F: CATTGGATGTGCCATAGTGC

R: AGGTATGCCCAAGGAAAGGT

55.2

53

388

TC

46

380–400

P

2

12

CNMK4627

F: TGCGTGACCAACTACTCGAT

R: GGAGCATGATGGGAGAGAAA

55.25

51

237

AG

56

220–245

P

4

13

CNMK4772

F: TGCATCAAGACAGACCATCC

R: TGCATGAATACACCCACATT

53.5

59

351

GT

59

380–450

P

3

14

CNMK4830

F: AAATGGCTTTGCATGTCTCC

R: GGTTGTCAAGCACCTTCCAT

54.75

53

341

AG

60

320–400

P

2

15

CNMK4976

F: TGCCACCATATGAGCAGAGA

R: CCCAGAGCTCCAAACTCATC

55.65

58

199

AC

64

170–200; 215–250

P

6

16

CNMK5103

F: AACGTGTCCACTCTCCCAAG

R: TTACCCGCCCTTATCTTCCT

56.15

54

307

CT

65

300–350

P

3

17

CNMK5211

F: AAGCTGACAAATGTGAAGAGC

R: CAACAACGGTCTAGCACTGG

54.75

51

388

CT

68

190–210; 242–310

P

6

18

CNMK5746

F: CTGGGCAATGACACAACAAG

R: AACCGACACAGAGAGGATGG

55.45

50

152

CT

80

380–400

P

4

19

CNMK5910

F: CGATTGAAGCTGATGCTCTG

R: TTGATGTGCGTGAATGGAAT

53.25

53

338

AG

82

330

M

1

20

CNMK6063

F: CCACAACATGAAAGCAAGGA

R: TGGACTAGGAGTGGGATTGG

54.7

53

394

AC

86

390–450

P

2

21

CNMK6206

F: TGCTAAAGGACCGGAAGATG

R: TCATGGAGAGGTGCATGTGT

55.4

53

321

GT

89

300–340

M

2

22

CNMK6376

F: CATCTCCTAAGCTGGCAACC

R: TAGGATAGGTGACGGCAACA

55.6

53

368

CT

92

380–450

P

3

23

CNMK6463

F: GATACAGGTGGCCAGGTGAG

R: TCCAGCACTCTGTGCGTTAT

57

57

304

GA

96

230–240; 300–320

P

4

24

CNMK6507

F: CCCAATACCTTTGGTTGCTC

R: TGCATGTCCGTGCATAAG

53.4

51

336

GA

98

150–160; 180–200

P

5

25

CNMK6571

F: TGAATGCCGTTGTTTGTACG

R: GGATAAGGTCTGCATGGCTAA

54.15

62

398

CT

99

400–450

M

1

26

CNMK6672

F: ATATAGGCAAGGCCCAAGGT

R: TGGTCATGGTGGAGTAGCAA

56.05

56

363

GA

102

330–370

P

4

27

CNMK7007

F: GGTGATTTCGTCGTCTCGTT

R: TGCAGAATGCTTGGCAATAG

54.3

57

381

TG

109

350–380

P

2

28

CNMK7162

F: ACGCACACCTGAAAGGTACA

R: GTTGCGAAGGATTTGCACTC

55.65

56

259

GT

113

250–320; 400–420

P

3

29

CNMK7449

F: TGCATCACACAGCTACACCA

R: GTGCAGTTTGCTGGTCTTCA

56.3

54

246

TC

119

240–260

P

4

30

CNMK7553

F: TTGAGGTGTTCCAAACATGG

R: GCTTGTAGGGCACGTTCATT

54.5

50

267

AG

121

240–260

P

3

31

CNMK7710

F: TCAACTGCATCAGGTCTTGG

R: CTGACAGGTGGCACAGAGAA

56

54

301

GA

127

220–230

P

3

32

CNMK7859

F: ATGGTCTGGATATGGCTTGC

R: TCTCCGTTCACTCTGCAACA

55.65

54

355

AG

132

300–310

P

2

33

CNMK8015

F: GGCAGTTCCACTTACCCAAG

R: TGCTTAACAAAGCGTTCGTG

54.9

55

391

TG

135

250–320; 400–480

P

2

34

CNMK8444

F: GGTCCGGGATTCAGGTTAGT

R: AGCCAAAGAACCCTTGGAAT

55.5

54

295

GT

152

200; 250–320

M

1

35

CNMK8741

F: ACCCGAGGTTTGAAAGGAAC

R: TTGGCACCTTCACTTATTGC

54.35

57

248

TC

166

320

M

1

36

CNMK9091

F: GCTTGATTCCCTGGATACGA

R: CTTGCTCTGTTCCCCATGAC

55.1

58

373

AG

185

320–380

P

2

37

CNMK9331

F: ATGCTTCGCTTGGTTGTAGG

R: AGTGAGGAATCCGATGCAAT

54.8

45

374

GA

196

350–380

P

2

38

CNMK9514

F: TGAGATGAGATGGGTGGACA

R: ATCAATGGGAGGTCACAAGG

54.95

50

324

TG

200

150; 220–240

P

3

39

CNMK9655

F: TTGGTCTAGTCCTGCCATAGG

R: CCAATCAACACCCACATTGA

54.55

50

381

AG

207

242–280; 370–400

P

2

40

CNMK9918

F: TTGGACTCCCAACGACTAGG

R: TTCCTTCCAAGCAGATGTCC

55.5

56

254

GT

224

230–242

P

2

41

CNMK10005

F: TGGTGCAGTCTTCTCAATCG

R: CCATCTTCTCCCTGATTCCA

54.35

54

251

CT

229

242–260; 320–350

P

2

42

CNMK10146

F: AATCGAAATACGTGGCGAAC

R: GCTTGTAGCAACTCCAACGTC

54.9

58

208

GA

237

210–225; 240–250

P

3

43

CNMK10298

F: TCACTCCATCACCCAAAGAA

R: TTTAGTCCCAAGTGCCCATC

54.2

54

237

AG

245

230–245

P

2

44

CNMK10608

F: AGGAACTCATCGGTGTTTGG

R: GCATGATTGTTGCATTGGAG

53.85

53

389

GA

265

350–380

P

2

45

CNMK10723

F: GTTTGCAGGTGGAAAGTCGT

R: AGCTTCTTGATGCCATAGCC

55.55

56

324

TC

274

280–350

P

3

46

CNMK10821

F: AATACGCCACGTTACCCTTG

R: TGCAGTGTGGAAGACACCAT

56

59

326

AG

280

310–350

P

3

47

CNMK11095

F: GATCGGCACTAGGGAACTTG

R: AATGCGAGGACAACTGGAAC

55.4

55

361

AG

295

330–360

P

2

48

CNMK11349

F: ATGGCATTTGAGGATGAGAA

R: ACCGTTCTTTGGGAAATGTG

52.55

62

280

AC

308

200; 300–350

P

3

49

CNMK16404

F: GGAATCTGAAGCAGGGACAG

R: AGGGCATTGAAGAACAGCAC

55.6

54

334

AG

1122

300–400

P

3

50

CNMK16553

F: GCCGAAATTGTCTTAATAGGTG

R: AGGAATGCCATGTCAGGTTC

53.45

48

339

GA

1168

200; 320–340

P

3

51

CNMK16634

F: CGAGCTTGAAGTCAGCTTTG

R: GCAGCCTTACCTCTCACGTC

55.95

54

357

AG

1203

400–500

P

2

52

CNMK17050

F: ATTGGCTGAGTGGAGGACAA

R: ATGAGCAACCCATGTTGATG

54.85

52

397

CT

1370

400–470

P

2

53

CNMK17101

F: CCAGCCATGCTTACCAACTTA

R: CGAGAACCACGTCAATGAGA

54.9

50

299

AG

1401

250–290

P

3

54

CNMK17156

F: GTGTTCTGGCAATCATGCTC

R: TGCTTGACATACGCACACAA

54.7

53

293

GA

1418

240–265

P

3

55

CNMK17229

F: CAAGCTGGAGACAACACAGG

R: TGTCATCGACGAACTGGAGA

55.75

56

400

AC

1462

280–320

P

2

56

CNMK17487

F: TGCCAATGTGTTAAGGATGC

R: TCCATACGAAGGCAACTGTG

54.25

52

288

CT

1550

310–350

P

3

57

CNMK17639

F: AAATCTGGGTGGCTCTCTCA

R: GCCAGCAGCTATGGAAGAAG

55.85

54

303

TC

1621

280–340

P

4

58

CNMK17725

F: GCTGAGTTGCTTGTTGTCCA

R: CCTGAACCAAGGGATGAGAA

55.1

55

234

AG

1655

170–190; 220–250

P

6

59

CNMK17797

F: GGTGCCTTAGTGCCTTCTCTT

R: AATCCGTTGCGACGTTATTC

55.25

53

370

GA

1688

315–350

M

2

60

CNMK17875

F: GGCTTGGGGTTCAAATTCTT

R: TTGAAGGCACCTAAGGCACT

55.1

50

397

AG

1750

300–400

P

2

61

CNMK18331

F: TGCAAAGTAAGGACCCTGGA

R: CTGATTCATGGTTGGCTCCT

55.45

55

368

AG

1987

320–500

M

1

62

CNMK18501

F: CTGGACGAAACAATGGTCCT

R: CTCCAGAGGGTATCCATCCA

55.25

55

380

TC

2084

350–400

P

2

63

CNMK18573

F: TGTGTTCGACTCGGTCGTTA

R: AGGCCTTCTTCGATCACTAGA

55.45

57

383

TG

2130

400–440

P

2

64

CNMK18799

F: GCCTGCATTATTCACCTGGA

R: GTCAGGAGGCAGTGGAGAAG

56.25

56

255

AG

2306

240–320

P

2

65

CNMK18903

F: CAGGACTCGGGAGATAGCTG

R: TTGGCTGCTAATGTCTGCAC

56.2

56

396

AG

2370

350–400

P

2

66

CNMK18972

F: GGTGGTTGGCATCCTATGTT

R: TACGTGGGGACACCAAGAGT

56.6

59

358

TC

2409

230–260; 330–400

P

5

67

CNMK19118

F: TACCCATCCCACAAATGGAC

R: TCAGGGTGGCATGATGAATA

54.1

52

393

CT

2535

230–320

P

4

68

CNMK19193

F: ATGTTGTGGGGACGATGAGT

R: ACCTCGCATGAGTGAAACTG

55.75

54

398

TC

2585

360–400; 690

P

3

69

CNMK19386

F: AAGGGTTTGAGTTGGTGGTG

R: CCTAACCAGGCAAAGGACAA

55.25

52

354

AG

2748

300–330; 400–420

P

3

70

CNMK19611

F: ATCCATCCAATGCTATCAGG

R: GACCGCATTAGCTCTGGTACT

54.2

50

364

AC

2872

300–340

P

3

71

CNMK19799

F: CGTCTGGGATAGCCTTCAGT

R: CCAAGCAACGGAGAACTTG

55.2

55

337

TC

2988

320–410

P

3

72

CNMK20018

F: TGACAAGTTTCAGGGCATCA

R: TGCAGATCTTGCCAAACGTA

54.5

53

362

AG

3251

300–330

P

3

73

CNMK20227

F: GCAGCACACTCATGCAAAAT

R: TTAGTGGCGAGAGAGGTTGG

55.55

54

278

TG

3455

320–400

M

1

74

CNMK20739

F: ACACGATTGATGCATGAGGA

R: GCCATGAGCCCACATCTATT

54.6

58

270

AG

4154

250–330

P

3

75

CNMK21015

F: CATGCCATTTGTCAATCCA

R: TGCAGAGGAGTCCAGTGATG

54.05

57

333

CT

4606

130–160; 310–500

P

2

76

CNMK21174

F: CATGACTGACCGCTCTACTCC

R: TCCTAATCCTCCATGTTGCTG

55.65

62

392

AG

4907

380–400

P

4

77

CNMK21493

F: AGGCATAGTCTCTCGGCTTG

R: CAAGTGGAATTGCTCGTGAA

55

55

315

TC

5710

220–230; 320–400

P

2

78

CNMK318

F: GGCAAACCTTCCTAAATGACC

R: CTTTGTCCAGCCGTACCTGT

55.6

60

358

TG

1

310–330

P

2

79

CNMK425

F: GGATGTAGGTTGGGCTCTTG

R: GCCACTAGAGGGTCATTGGA

55.9

59

217

TG

2

130–160; 180–200

P

3

80

CNMK808

F: CCATGCCACACCTTACTCCT

R: AACAAACGCCCACCTATCTG

56.1

55

225

GA

5

280–320; 400–430

P

3

81

CNMK3765

F: GAGAGAGTGGTCGGCTTCAC

R: ATTCGGATGTTCGATTTGGA

54.7

55

372

TC

40

290–380

P

3

82

CNMK4127

F: TCAACGCATCAATACCCAAG

R: GAAGTCCAAGCAACCAGCTC

54.65

58

274

TG

46

160; 350–390

M

1

83

CNMK5054

F: CTTCATGGTTCATGGTGCAG

R: ATGGATATGAACAGCGAGCA

54.1

57

391

TG

64

400–440

P

2

84

CNMK5329

F: GCTGGTCGGAAATGCTAGAC

R: CATGCAAAGCCTCACTCAAG

55.05

53

320

ACAT

70

300–320; 360–410

P

2

85

CNMK5632

F: TAGCCCTTTCAGGACCCTCT

R: GAGGAAGTCATCCGAAGCAG

56.4

56

180

TG

78

180–200

P

5

86

CNMK6746

F: AAGCACTTCCTTACGCCAGA

R: GCCTTGGTGGTGAAGATTGT

56.05

59

382

TG

103

380–400

P

2

87

CNMK6908

F: AGATTGCCGGAGTTGATTTG

R: CCTATTCGGTCGCAATTGAT

53.05

58

344

CT

107

200–300

P

3

88

CNMK7627

F: TTGAGTCTGGAATCGTTAGAGG

R: CATGGTGGCGAACTGTGATA

54.45

54

356

TG

124

240–350

M

1

89

CNMK7985

F: GAAATGAGACCGCCATTGAT

R: CGGACCGTTAGACAGATTGC

54.4

57

323

AC

135

320–340

P

2

90

CNMK8083

F: GGCGTATTCGGTAGCATCTC

R: CTCCAGCACAGATGGAAACA

55.15

58

247

GA

137

200–250

P

3

91

CNMK8371

F: AAGGACTTGTGATGCCTTGG

R: GTCACCATAGCCGACAACCT

56.2

54

310

AG

148

310–400

P

3

92

CNMK8904

F: GTTTGCCCGTACTTCTCAGC

R: TGGCAGCATCACTCTTTCAC

55.85

56

364

GA

178

320–350

P

2

93

CNMK9440

F: TGGGACCTGTCCTGCATATT

R: TATCGGCACATTCGATTTCA

53.9

54

333

GA

199

300–340

P

2

94

CNMK9988

F: CTCGCAAATGCAATATAGGC

R: CGCAAATTCGGTTGATCTTA

51.45

49

383

GA

228

230–320

P

4

95

CNMK10263

F: TTCAGGACAATTGGAAGTGTTG

R: AGAATGCCCAAGTCAAGCAG

54.5

55

313

CT

244

290–320; 400–500

P

5

96

CNMK10632

F: TTCACGTTGCCAAATGACAC

R: CAGGCATGCACTCAAAGATG

54.25

52

376

AC

268

150

M

1

97

CNMK10681

F: CGACCTCCATACATGGCTCT

R: TAACTGGCTTTGGGTTGGTC

55.85

57

337

TG

269

320–400

P

3

98

CNMK11807

F: AGTGAAGATCTGCCCGAGAA

R: TTTGAACTCACGCTTGTTGC

55

55

305

CT

346

242–330

P

3

99

CNMK12241

F: AGTGCTAGCCAGACCCATGT

R: CTCCCAAAGGTACGTGCAAT

56.9

55

315

AG

385

240–330

P

4

100

CNMK12746

F: TAGATCGAGGCATGCGAGTA

R: AGAATGGATATTGGCCCTCA

54.3

54

383

TG

441

380–450

P

2

101

CNMK13043

F: CCTTCAGGGTTAGGTGCAGA

R: TCAAACTGGCTGATCCTTCA

55.25

55

383

AG

472

320–340

P

3

102

CNMK13232

F: GGAAGTCCTCAGTCGTGCTC

R: GGAAATCAAGAAGGCATTGG

54.7

58

165

TC

495

170–230

P

3

103

CNMK13632

F: CGAGGGTCTCAGCGATTTAG

R: GCTGGACCTTTGTGGTGAAG

57.2

57

379

TG

561

300–330

P

3

104

CNMK13852

F: TTGGAAGAAATGGCAGTGGT

R: GGATATGGATGGATGGATGG

53.55

52

184

AG

595

390–450

M

2

105

CNMK13946

F: GGCAGAGGTAGTGGAACGAG

R: CCTGACGGAGGACTGTTGAT

56.9

57

363

TC

605

330–400

P

3

106

CNMK14272

F: AAGGGTGCATGATGGTTAGG

R: CAAACATTCCTCCGTGTGTG

54.55

53

371

AG

643

320–410

P

2

107

CNMK14692

F: GGAGGCTACCAGCCATAGTG

R: CACAGTCCTCTGCGATGAGA

56.85

53

370

GA

700

330–380

P

2

108

CNMK15137

F: TTGGTCGCATGATTGTCTTC

R: CTGAGCACCCTGTGGTAGGT

56.1

56

337

TC

796

330

M

1

109

CNMK15508

F: ATAGCTGGTGAGTCGGCAAG

R: GGCCTACTGATTGGACTGGA

56.75

57

374

AG

868

200–220; 320–400

P

3

110

CNMK15694

F: AAGACTGTTGCCCTGGAAGA

R: TCGATGATGCAGAGATCAGG

55.15

55

353

AG

908

320–390

P

3

111

CNMK15970

F: CGTGTTGGTGATTGTTGCAT

R: ATTGCGGGGTAAGGAGAAGT

55.15

53

294

TG

994

220–280

P

3

112

CNMK5852

F: ACCCACTAGCACTTGCACCT

R: CCTGAGGTCAACAAGCCATC

57.4

60

310

AG

82

310–400

P

3

113

CNMK17532

F: AAGTTCGGCTCACCAATCAC

R: GATGGGGATACATCCAATGC

54.4

55

388

AG

1570

350–500

P

2

114

CNMK17684

F: TAGCCGTCCGATATTCAAGG

R: TGCATTCTAAGGGAATGGATG

53.3

53

176

CT

1636

170

P

2

115

CNMK18364

F: TCCCAATGGCAGTCCTAGTC

R: GAAACCCATCCTTGTGGAGA

55.45

58

347

GA

2021

260–320; 470

P

4

116

CNMK19333

F: ACCTGCCTATTCATGCACAA

R: GAGTGACGCAAGACAATCTCC

55.15

53

304

AC

2726

280–420

P

4

117

CNMK19929

F: AACTGAGCAGATGGGCTTTG

R: AGCCTCTGTGACGAACGAAT

56

56

321

AG

3153

260–330

P

3

118

CNMK20075

F: GTTGTGCCTCCAATGTTCCT

R: GATCGGCCTGAATCCTGTAA

54.95

55

280

GA

3333

300–320

P

4

119

CNMK1433

F: GGTGATTGACTCCTGGCACT

R: TTCACCTCTGGATTCTTGGTG

55.85

50

183

GA

10

150–170

M

1

120

CNMK1524

F: GCTCCTGGTACAGGCACATA

R: ACATCGGGATGGGTTCAAT

55.3

55

266

GA

11

230–250

M

1

121

CNMK1644

F: TAGGACGTTTACCGCAGGAG

R: CTGTAGGGTAGGGTGCATCG

56.8

57

205

TC

12

190–210; 270

P

4

122

CNMK1809

F: CGGGACTTGGGAGTCATCTA

R: TGGCACTTCGTCTGTGTAGC

56.6

57

386

AG

14

350–500

M

1

123

CNMK2363

F: CAAGACACAGCTTCGAGATCC

R: GATTCCTCCGCCTATGACCT

55.85

56

400

AG

22

350–380

M

1

124

CNMK2470

F: GCAATCGAGCCCAGAACTAC

R: CCCAACCTTCCACCAATATG

54.6

55

369

CT

23

250–300

M

1

125

CNMK2651

F: CCTCCCTTCACCTTGCATAA

R: CTGCACTGCTCACCGTATGT

56.05

54

306

GA

25

280–320

P

3

126

CNMK2960

F: GAGGAGTGAGACGGATTGGA

R: CGATCCAAGATTGGTACTGGA

55.05

55

300

CT

30

260–300

P

3

127

CNMK3730

F: CGATTGAAGCCCAGTCTCTC

R: AACGACATCTTCACCAGCAA

54.95

53

296

AAG

40

250–280

P

3

128

CNMK3865

F: AGGAGTAGCTCCGCCCTCTA

R: CCCTCGAATGACCAGAGAAG

57.05

57

323

AG

43

300–320

P

2

129

CNMK4080

F: AGTTTCGTAGCGGCTGATGT

R: AGAGCACTCAGCAAGCAGGT

57.9

53

327

AG

45

300–320

P

4

130

CNMK4336

F: CCGACGTGTTGACAGCTCTA

R: AAACCTTTCGCACGAATCAC

55.35

55

229

AC

49

230–300

P

4

131

CNMK5287

F: CCCAACAGACCCAACTCAAT

R: TGTGGAAGATGTGGAGTGGA

55.4

53

205

CT

69

230–340

P

4

N/A

N/A

N/A

N/A

N/A

N/A

N/A

2.7