Table 4 siRNAs designed in the present study for the “S” gene and various parameters
siRNA | Conserved region ID | Target sequence (21 + 2 nt) | siRNA sequence antisense/guide) 21 nt | Sense/passenger (19 nt) | SMEpred (efficacy) | Free energy of binding | Free energy of folding | Whole dG (kcal/mol) | GC content (%) | siRNA scales | RNAxs (position) | OligoWalk (probability value) | Guide (Tm) | Passenger (Tm) | siDirect (position) | i-Score |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S10.3 | 10 | AAGGAATCTATCAAACTTCTAAC | UAGAAGUUUGAUAGAUUCCuu | GGAAUCUAUCAAACUUCUA | 100.7 | −30.6 | 1.5 | −31.9 | 31.6 | 7 | 67 | 0.90315 | 17.7 | 16 | 47–69 | 79 |
S28.5 | 28 | AGCTGTTGAACAAGACAAAAACA | UUUUUGUCUUGUUCAACAGcu | CUGUUGAACAAGACAAAAA | 97.3 | −31.2 | 1.6 | −30.3 | 31.6 | 11 | 113 | 0.893136 | 14.9 | 20.5 | 93–115 | 72.7 |